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INDONESIA
Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 8 Documents
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Search results for , issue "Vol 21 No 2 (2016): June 2016" : 8 Documents clear
Study of adhesin from Aggregatibacter actinomycetemcomitans local isolate on alveolar bone destruction in aggressive periodontitis disease Rini Devijanti Ridwan; Tuti Kusumaningsih; Sidarningsih Sidarningsih; Soetjipto Soetjipto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 21 No 2 (2016): June 2016
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.894 KB) | DOI: 10.23869/75

Abstract

Adhesion is a powerful survival mechanism as well as a virulence mechanism for bacterial pathogens. Bacterial adhesin is a media for bacteria to invade the host. Bacterial adhesin,is a medium for bacteria to invade the host. Baterial adhe-sion, moreover, is depend on the ligand interaction as a signaling mediator that will influence the invasion process and increase pro and anti-inflammatory due tob the influence of the receptors of innate immune response. Aggregatibacter actimycetemcomitans (A.actinomycetemcomitan) have many virulence factors that may result in tissue and alveolar bone damage. One of the virulence factors is adhesin that can be isolated from the fimbriae. This research purposed to analyze the ability of adhesin protein from A.actinomycetemcomitan that cause the destruction of alveolar bone. Thus, the number of osteoblasts and osteoclasts as well as osteocalcin expression can be used as a marker of damage on the alveolar bone of Wistar rats. The research was conducted through several processes. First, the adhesin of A.actinomycetemcomitan with a molecular weight (MW) of 24 kDa is induced into Wistar rats. Next, to determine the number of osteoblasts and osteoclasts performed, hematoxylin eosin staining is conducted. Meanwhile, to determine osteocalcin expression performed, immunohistochemical techniques is used. This research shows the decreasing of the number of osteoblasts and increasing of the number of osteoclasts in the treatment groups induced by adhesin proteins, A. actinomycetemcomitans + adhesin protein, and A.actinomycetemcomitan compared those in the control group. It also shows the increasing of osteocalcin expressions on the alveolar bone of Wistar rats in the groups induced by adhesin proteins, A. actinomycetemcomitans + adhesin protein, and A. actinomycetemcomitans than those in the control group. It can be concluded that the adhesin protein of A. actinomycetemcomitans plays an important role in the destruction of alveolar bone through the reduction of the number of osteoblasts, the increasing of the number of osteoclasts and oste-ocalcin expression in aggressive periodontitis.
The effect of polysaccharides krestin bioactivity from coriolus versicolor to estradiol levels on Mus musculus estrous cycle Sri Puji Astuti Wahyuningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 21 No 2 (2016): June 2016
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (543.451 KB) | DOI: 10.23869/76

Abstract

This study aimed to determine the activity of polysaccharide krestin (PSK) from Coriolus versicolor extract to the dura-tion of estrous cycle and estradiol levels. PSK was administrated for 24 days in female Mus musculus, strain Balb/C, 10 weeks old, weight about 25-30 grams. Polysaccharide krestin given in sub-chronic dose. There were 4 groups, i.e K0 (0 mg/kg BW), P1 (15 mg/kg BW), P2 (30 mg/kg BW), and P3 (60 mg/kg BW). Each treatment group contained 7 replica-tions. Data were analyzed using One Way ANOVA and Duncan test at α = 5%. The duration of estrous cycle was de-termined by vaginal smear methods. The estradiol levels were measured using ELISA kit. The results show that the administration of PSK decreased the duration of estrous cycle with an average of 4.4 days. Polysaccharide krestin dose of 15 and 60 mg/kg BW did not affect the estradiol levels. Decrease of estrous cycle duration was still in the range of normal estrous time between 4-6 days. PSK dose of 15 and 60 mg/kg BW can be used as a therapeutic dose.
Histologic profile and CD44 expression in breast cancer tissue of balur nano divine kretek volunteers Dody Novrial; A’liyatur Rosyidah; Saraswati Saraswati; Subagjo Subagjo; Sarjadi Sarjadi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 21 No 2 (2016): June 2016
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (907.437 KB) | DOI: 10.23869/77

Abstract

Breast cancer is the most common cancer in woman. Accumulation of free radical in the body is one of cancer risk fac-tors. Since 2009, Research Institute for Free Radicals Shedding Malang has been developing balur nano divine kretek method, combination of balur and divine tobacco smoke to decay free radicals. It has been tested on volunteers with cancer, including breast cancer. This study aimed to assess the effect of balur nano divine kretek method to breast can-cer tissue and CD44 expression. The results showed that 66.67% tumor samples suffered extensive damage (grade 3) and high CD44 expression. The high expression of CD44 in most of the samples may indicate better prognosis. The augmentation for the effectiveness consistency of balur nano divine kretek should be continuously conducted with greater samples and more complete molecular markers such as CD24 and ALDH.
Antimicrobial activity of Streptomyces spp. isolates from vegetable plantation soil Isnaeni Isnaeni; Idha Kusumawati; Mega Ferdina Suwito; Asri Darmawati; Ni Made Mertaniasih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 21 No 2 (2016): June 2016
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (639.33 KB) | DOI: 10.23869/78

Abstract

Fifteen Streptomyces isolates were isolated from soil in some different location on vegetable plantation at agriculture standard condition. The isolates were assessed for their antibacterial activity against Mycobacterium tuberculosis (MTB) ATCC H37RV and mycobacterial which isolated from Dr. Soetomo Hospital patients in Surabaya. The Interna-tional Streptomyces Project 4 (ISP4) and Middlebrook 7H9 (MB7H9) wwere used as growth or fermentation medium. The screening of inhibition activity was performed using turbidimetry and spot-test on agar medium. Results shown that 33.3% of the isolates (5 isolates) have anti-mycobacterial activities. The first line anti tuberculosis drug rifampicin, (RIF), ethambutol (EMB), isoniazid (INH), and pyrazinamide (PZA) were used as standards or positive controls with concentration 20 ppm. Optical density of crude fermentation broth concentrated from five isolates relatively lower than five anti-tuberculosis drug activity standard, although their activities against some microbial were similar to the stand-ard at spot-test. The most efficient isolate shown anti-mycobacterial activity was Streptomyces B10 which identified as Streptomyces violaceousniger. In addition, fatty acid methyl ester (FAME) profile of gas chromatography-mass spec-trometry chromatogram of each isolates were studied and compared to Streptomyces spp.
The exploration of fruit flies Bactrocera (Diptera:Tephritidae) and its parasitoid in Madura Island Regions Tjipto Haryono; Ika Rochdjatun Sastrahidayat; Gatot Mudjiono; Toto Himawan
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 21 No 2 (2016): June 2016
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (570.199 KB) | DOI: 10.23869/80

Abstract

Madura is enriched by great diversity despite of its infertile natural condition. This condition influences fruit flies existence and diversity. Purpose of this study was to investigate the diversity and distribution of fruit flies with their host in Madura region. Sampling methods in this study were fruit host collection (rearing) and trapping using Steiner-type trap that were set in 48 locations in several villages in Bangkalan, Sampang, Pamekasan, and Sumenep regencies. Steiner traps were combined with 2 different attractants, such as methyl eugenol (ME) and Cue Lure (CL). There were 5 species of fruit flies obtained from trapping and rearing, namely Bactrocera carambolae, B. papayae, B. umbrosa, B. albistrigata, and B. cucurbitae. Results indicate that the distribution, diversity, and abundance of fruit flies were influenced by the diversity of fruit host, air temperature, and relative air humidity. It is also identified two species of parasitoid imago from rotten fruits collection, namely Biosteres vandenboschi and Fopius arisanus.
POPULATION DYNAMIC OF Viscum articulatum Burm. f. ON ITS HOST IN PURWODADI BOTANIC GARDEN Solikin Solikin
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 21 No 2 (2016): June 2016
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (533.629 KB) | DOI: 10.23869/81

Abstract

V. articulatum Burm. f. is a parasitic plant that has potential as medicinal plant. The research aim is to study the population dynamic and hosts of V. articulatum Burm. f. in Purwodadi Botanic Garden which was conducted in April-September 2013 by cruising method and literature study on previous research of parasitic plants in Purwodadi Botanic Garden. The results show that V. articulatum Burm. f. found living as hyperparasite on the parasite Dendrophthoe pentandra (L.) Miq. The population number of V. articulatum Burm. f. increased following the increasing of host and time. The species, genera and family of the host trees increased from 20 species, 14 genera and 13 families in 2005 to 23 species, 19 genera and 17 families in 2013.
FOLIAR SYMPTOMS RECOVERY: DEVELOPING SCORING TECHNIQUE FOR ASSESSMENT OF SOYBEAN RESISTANCE TO CPMMV (COWPEA MILD MOTTLE VIRUS) Siti Zubaidah; Heru Kuswantoro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 21 No 2 (2016): June 2016
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (568.171 KB) | DOI: 10.23869/82

Abstract

Soybean is a commodity that has an important role as the source of protein, but its production is affected by various factors including disease. CpMMV (Cowpea mild mottle virus) is one of the most damaging viruses that cause soybean disease. CpMMVs belong to the group of Carlavirus that are transmitted by whitefly (Bemisia tabaci). The use of CpMMV resistant plants as biological control can prevent viral diseases. Indonesia has many soybean germplasm from many regions and introduction from other countries that need to be evaluated for CpMMV. The assessment technique for soybean resistance to CpMMV is not available yet, but it is still based on other virus diseases. The specific assessment of plant resistance is important because some diseases can cause certain symptoms, depends on the resistance and the kinds of plants that are infected. This paper presents the assessment technique for soybean resistance to CpMMV infection, which can be used for various purposes and studies. One of the benefits is to find out soybean resistance to CpMMV or other objectives. This resistance assessment is not only based on leaves symptoms, but also based on the phenomenon of foliar symptoms recovery.
Endophytic fungi colonize agricultural and non agricultural plants in Bedugul, Bali and their antifungal activity against Rhizoctonia solani Kuhn Suciatmih Suciatmih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 21 No 2 (2016): June 2016
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (434.731 KB) | DOI: 10.23869/85

Abstract

Isolation of endophytic fungi was done to find alternative microorganisms as antifungal agent against Rhizoctonia solani Kuhn, a soil borne pathogen on many agricultural plants. The research objectives were 1) to isolate and identify endophytic fungi colonize agricultural and non-agricultural plants growing in Bedugul, Bali; and 2) to detect for their antifungal activity against R. solani under in-vitro conditions. The results indicated that 114 isolates of endophytic fungi were isolated from flowers, fruits, leaves, petioles, and stems of agricultural and non-agricultural plants. Ten isolates (8.8 %) were identified to species, 91 isolates (79.8 %) to genus, and 13 isolates (11,4 %) did not have spores that could not be identified morphological characters and classified as unidentified isolates. Endophytic fungi isolated including in group of Aspergillus, Bipolaris, Cladosporium, Colletotrichum, Corynesporopsis, Curvularia, Diplodia, Fusarium, Guignardia, Nigrospora, Pestalotiopsis, Phomopsis and Xylaria. Of the 114 fungal isolates tested, only 13 isolates (11.4 %) inhibited the growth of R. solani from 10.3 % to 62.2 % with a percent inhibition. The highest growth inhibition of R. solani was shown by Aspergillus niger isolated from Solanum licopersicum L. var cerasiforme (62.2 %). It could be concluded that the agricultural and non-agricultural plants growing in Bedugul, Bali were colonized by endophytic fungi. Aspergillus niger will be further examined on a field scale.

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